BBa_K152001

BBa_K152001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K152001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Johnston Monje
Date created: 2008-10-29 12:00:00
Date modified: 2015-05-08 01:10:49

rbs + crtE



Types
DnaRegion

Roles
Translational_Unit

engineered_region

Sequences BBa_K152001_sequence (Version 1)

Description

crtE from Erwinia uredovora with a rbs preceeding it. This gene codes for geranylgeranyl diphosphate synthase which is the first committed step in the beta carotene synthesis pathway.

Notes

We wanted to use this in synthetic operon construction, and as the first gene in the transcript, so we included a NdeI restriction site at the start codon.

Source

The plasmid pAC-3.10.10.A which was sent to us by Ethan Johnson on iGEM Minnesota team timebomb.

Sequence Annotation Location Component / Role(s)
rbs
cds
3,10
18,926
feature/rbs ribosome_entry_site
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K152001/1