BBa_K152003

BBa_K152003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K152003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Johnston Monje
Date created: 2008-10-30 12:00:00
Date modified: 2015-05-08 01:10:49

CrtY + RBS



Types
DnaRegion

Roles
engineered_region

Translational_Unit

Sequences BBa_K152003_sequence (Version 1)

Description

crtE from Erwinia uredovora with a rbs preceeding it. This gene codes for lycopene cyclase which is the third step in the beta carotene synthesis pathway. In combination with CrtE and B, this works to produce lycopene, a red, antioxidant, carotenoid.

Notes

We needed to use site directed mutagenesis to add the SpeI site after the stop codon.

Source

The plasmid pUC-EUY which was sent to us by Ethan Johnson on iGEM Minnesota team timebomb.

Sequence Annotation Location Component / Role(s)
rbs
cds
4,10
18,1166
ribosome_entry_site feature/rbs
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K152003/1