Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: David Johnston Monje
Date created: 2008-10-30 12:00:00
Date modified: 2015-05-08 01:10:49
TB1 RNAi construct
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K152010_sequence (Version 1) |
Description
This is meant to be cut out using NdeI and PstI for ligation downstream of the strong promoter pDSK-GFPuv, followed by electroporation into the corn endophyte Klebsiella pneumonii 342. The expected goal is to see transcriptional knockdown of the gene in the plant when these bacteria are injected into it.Notes
This part MAY have been made incorrectly (the antisense fragment got inserted in the sense orientation by accident). Stay tuned or try it out yourself to see what's up.Source
Mix of two biobricks| Access | Instance | Definition |
| public public | BBa_K152008 BBa_K152009 | BBa_K152008 BBa_K152009 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K152008 BBa_K152009 | 1,232 241,465 | BBa_K152008 BBa_K152009 |