BBa_K1526000

BBa_K1526000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1526000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Teodora Manea

Date created: 2014-10-04 11:00:00

Date modified: 2015-05-08 01:10:50

SpPCS: Phytochelatin synthase from Schizosaccharomyces pombe

Details

• Types: DnaRegion
• Roles: Translational_Unit; engineered_region
• Sequences: BBa_K1526000_sequence (Version 1)

Description

The phytochelatin synthase in S. pombe uses glutathione with a blocked thiol group in the synthesis of phytochelatin. Overexpression of this gene, together with Gsh1* (BBa_K1526001), in E.coli has been shown to increase phytochelatin production and lead to a 7.5-times-higher Cd accumulation (http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2075016/).

Notes

Codon optimized sequence for E.coli of the S.pombe phytochelatin synthase gene

Source

NCBI Reference Sequence: NM_001018985.2

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_B0034	7,18	ribosome_entry_site, feature/rbs
Start	25,27	feature/start, start_codon
Stop	1267,1269	stop_codon, feature/stop
SpPCS	25,1269	CDS, feature/cds

Other Properties

• igem#experience: None
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1526000/1

Member of these Collections

• iGEM Parts Registry