Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xinyuan Qiu
Date created: 2014-09-28 11:00:00
Date modified: 2015-05-08 01:10:51
Cla I cutting site (methylation insensitive)
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K1532000_sequence (Version 1) |
Description
This part is the cutting site of the restriction endonuclease Cla I.The endonuclease Cla I can specifically recognize the sequence of this part and cut the double helix into two cohesive ends.
You can use this part when you are trying to add an extra cutting site inside of your system in order to make it open-edited.
Notes
The original cutting site is sensitive to methylation, we re-engineered the sequence so that the methylation will not affect the cutting of Cla I.Source
The coding of the Cla I cutting site is open-accessed.| Sequence Annotation | Location | Component / Role(s) |
| ClaI cutting site | 3,8 | non_covalent_binding_site feature/binding |