Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jie Li
Date created: 2014-08-17 11:00:00
Date modified: 2015-05-08 01:10:52
35S promoter+translation initiation optimized sequence for dicot+Mass translation enhancer
| Types | DnaRegion |
| Roles | promoter Regulatory |
| Sequences | BBa_K1537015_sequence (Version 1) |
Description
The 35S promoter is a strong promoter derived from cauliflower mosaic virus. This constitutive promoter is widely used in transgenic plants to improve the level of the expression of foreign genes effectively."AAAAAAATG" was a kind of preferred nucleotide sequence used in dicot plants（ATG is the initiation codon）to enhance translation initiation.And insertion of ???GCT TCC TCC???(mass translation enhancer) after the initiator codon ATG can augment downstream gene-expression in plants.Notes
We have to remove some restriction endonuclease cutting sites by changing a few bases.Source
The 35S promoter has a large area of application in genetically modified plants.The translation initiation optimized sequence for dicot is from the paper "translation initiation optimized sequence for dicot"(Nucleic Acids Research Volume 36, Issue 3Pp. 861-871 So Nakagawa1, Yoshihito Niimura2, Takashi Gojobori3,4, Hiroshi Tanaka1,2,* and Kin-ichiro Miura5)| Sequence Annotation | Location | Component / Role(s) |
| 35s promoter translation initiation optimized sequence for dicot mass translation enhancer | 1,842 843,851 852,860 | promoter feature/promoter engineered_region feature/BioBrick engineered_region feature/BioBrick |