LE Promote

BBa_K1539089 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1539089
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Coleen Tran
Date created: 2014-10-09 11:00:00
Date modified: 2015-05-08 01:10:52

LE Promoter Primer



    • Details

    Types
    DnaRegion

    Roles
    primer

    Primer

    Sequences BBa_K1539089_sequence (Version 1)

    Description

    This primer can be used to insert a low efficiency promoter to translate E. coli. genes. Must be used after insertion of RBS primer. Use Georgia Tech iGEM RBS primers (BBa_K1539021 or BBa_K1539034) or an RBS primer with a similar design.

    Notes

    Primer design could not retain the EcoRI site but once the construct (promoter->RBS->gene) is reinserted into pSB1C3 backbone, EcoRI is site is reestablished.

    Source

    Primer was ordered from IDT.

    igem#experience
    None
     
    igem#sampleStatus
    Not in stock
    igem#status
    Unavailable
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1539089/1
     

    Attachments

    © 2018 Newcastle University, University of Utah, and collaborators
    About SynBioHub | View Source on Github | Report an Issue
    | v1.6.1 (57f00336)