BBa_K1550001

BBa_K1550001 Version 1

Component

This part has been discontinued.

Source:
http://parts.igem.org/Part:BBa_K1550001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jesse Goldenberg
Date created: 2014-10-16 11:00:00
Date modified: 2015-05-08 01:10:53

Metallothionein with RBS and tt



Types
DnaRegion

Roles
engineered_region

Composite

Sequences BBa_K1550001_sequence (Version 1)

Description

This part includes the strong RBS BBa_B0034 (Elowitz, 1999), BmtA from Oscillatoria brevis, which encodes lead binding metallothionein (Liu et al., 2003), and the double transcription terminator BBa_B0015. The BmtA sequence in this part has been codon optimized for E. coli. This part can be used with a constitutive or inducible promoter to produce metallothionein in projects utilizing the metal-binding protein for sequestration purposes.

Notes

When used in E. coli for the sequestration of lead or zinc ions in solution, the part may or may not work better in concert with a genomic ZntA knockout. We plan to characterize this combination in the near future.

Source

The sequences for pre-existing basic parts (BBa_B0034 and BBa_B0015) were incorporated as found in the iGEM registry. The sequence used for the BmtA (metallothionein) was codon optimized for E. coli using the browser-based software, Gene Design. The native sequence comes from Oscillatoria brevis, and was sourced from an entry in the NCBI database by Liu,T., Nakashima,S., Hirose,K., Uemura,Y., Shibasaka,M., Katsuhara,M. and Kasamo,K. (2003).

igem#experience
None
 
igem#sampleStatus
Discontinued
igem#status
Deleted
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1550001/1