Types | DnaRegion
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Roles | engineered_region
Device
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Sequences | BBa_K1554004_sequence (Version 1)
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Description
This biosafety device for plants consists on two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consist on a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the yellow chromoprotein AmilGFP, so that plants can be visually differentiated from non-transgenic plants.
Notes
The assembly was performed by GoldenBraid assembly. The barnase was ampliified by PCR from
Part:BBa_J72173 and a mutation in T105P was introduced.