BBa_K1554004

BBa_K1554004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1554004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Valencia_UPV team members
Date created: 2014-09-28 11:00:00
Date modified: 2015-06-09 09:45:45

Yellow biosafety module for plants



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K1554004_sequence (Version 1)

Description

This biosafety device for plants consists on two submodules: a male sterility submodule and an identity preservation submodule. The male sterility submodule consist on a barnase specifically expressed in anthers under the regulation of the TA29 tapetum-specific promoter. As result, pollen from these plants is not fertile. The identity preservation submodule expresses the yellow chromoprotein AmilGFP, so that plants can be visually differentiated from non-transgenic plants.

Notes

The assembly was performed by GoldenBraid assembly. The barnase was ampliified by PCR from Part:BBa_J72173 and a mutation in T105P was introduced.

Source

TA29 promoter Part:BBa_K1554000

Barnase Part:BBa_J72173

AmilGFP [Waiting for Norwich team part name in registry]

P35s Part:BBa_K1537015

Sequence Annotation Location Component / Role(s)
T35s
TA29 promoter
Barnase
T35s
P35s
AmilGFP
3166,3391
4,858
863,1195
1200,1425
1438,2464
2469,3161
stop_codon feature/stop
feature/promoter promoter
feature/cds CDS
stop_codon feature/stop
feature/promoter promoter
CDS feature/cds
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1554004/1