BBa_K156003

BBa_K156003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K156003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: George McArthur IV
Date created: 2008-10-27 12:00:00
Date modified: 2015-05-08 01:10:54

BioBrick placeholder 3 (Prefix - ApoI (NotI) NsiI - Suffix)



Types
DnaRegion

Roles
sequence_feature

DNA

Sequences BBa_K156003_sequence (Version 1)

Description

This BioBrick Placeholder part is one of a set of eight that represent a new technical standard useful in composite part construction. Flanked by the standard BBa restriction sites, these parts are made of internal restriction sites that are unique but compatible with the standard sites (see below). This allows insertion of a desired part (e.g., RBSs, coding regions) within a previously-made construct.

Compatible sites:

EcoRI - ApoI & MfeI

XbaI & SpeI - AvrII, NheI

PstI - NsiI, SbfI


Notes

Sites are compatible with standard BBa restriction sites and will form scars when ligated.

Source

The DNA sequence is simply linked restriction enzyme sites that are compatible with standard BBa restriction sites. These compatible sites were compiled and listed in "Engineering BioBrick vectors from BioBrick parts". http://www.jbioleng.org/content/2/1/5

igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K156003/1