BBa_K156005

BBa_K156005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K156005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: George McArthur IV

Date created: 2008-10-27 12:00:00

Date modified: 2015-05-08 01:10:54

BioBrick placeholder 5 (Prefix - MfeI (NotI) AvrII - Suffix)

Details

• Types: DnaRegion
• Roles: sequence_feature; DNA
• Sequences: BBa_K156005_sequence (Version 1)

Description

This BioBrick Placeholder part is one of a set of eight that represent a new technical standard useful in composite part construction. Flanked by the standard BBa restriction sites, these parts are made of internal restriction sites that are unique but compatible with the standard sites (see below). This allows insertion of a desired part (e.g., RBSs, coding regions) within a previously-made construct.

Compatible sites:

EcoRI - ApoI & MfeI

XbaI & SpeI - AvrII, NheI

PstI - NsiI, SbfI

Notes

Sites are compatible with standard BBa restriction sites and will form scars when ligated.

Source

The DNA sequence is simply linked restriction enzyme sites that are compatible with standard BBa restriction sites. These compatible sites were compiled and listed in "Engineering BioBrick vectors from BioBrick parts". http://www.jbioleng.org/content/2/1/5

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K156005/1

Member of these Collections

• iGEM Parts Registry