BBa_K157003

BBa_K157003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K157003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Freiburg 2008
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:10:54

Anti-NIP singlechain Fv-Fragment



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_K157003_sequence (Version 1)

Description

Singlechain Fv.Fragment of an Anti-Nitro-Iodo-Phenol-antibody B1-8. Binds the hapten NIP (Nitro-Iodo-Phenole) with an affinity of 2.0 uM[1,2]. Designed for fusion to proteins or peptides via in frame cloning.

Notes

Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.

Source

Gene synthesis by GeneArt, optimized for expression in homo sapiens.

Sequence Annotation Location Component / Role(s)
Anti-NIP sc Fv-fragment
1,735
feature/cds CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K157003/1