Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Freiburg 2008
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:10:54
Split-Cerulean-nCFP
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_K157006_sequence (Version 1) |
Description
N-terminal fragment (Amino acids1-155) of the monomeric[5] cyan fluorescent protein variant ???Cerulean???, designed for fusion/BiFC[1,3]. Reassembly with Split-Cerulean-cCFP (Part Bba_K157005) generates the complete, working CFP ???Cerulean???, whereas combination with the C-terminal fragment (Part Bba_K157007) of the monomeric yellow fluorescent Protein ???Venus???[4] (Part Bba_I757008) results in green fluorescence[2].- [1] Chang-Deng Hu, Yurii Chinenov, Tom K. Kerppola: ???Visualization of Interactions among bZIP and Rel Family Proteins in Living Cells Using Bimolecular Fluorescence Complementation???, Molecular Cell, Vol. 9, 789???798, April, 2002
- [2] Chang Deng Hu, Tom K. Kerppola: ???Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis???, Nat Biotechnol. 2003 May; 21(5):539-545 (doi:10. 1038/nbt816)
-[3] Tom K. Kerppola: ???Design and implementation of bimolecular fluorescence complementation (BiFC) assays for the visualization of protein interactions in living cells???, Nat Protoc. 2006;1(3):1278-1286 (doi:10.1038/nprot.2006.201)
-[4]Nagai, T. et al. ???A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications??? J. Biol. Chem. 276, 29188-29194, 2001
-[5]Roger Y. Tsien et al. ???Creating new fluorescent probes for cell biology???, Nature Biotechnology Reviews, Vol. 3, 906-918, 2002
Notes
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.Source
Gene synthesis by GeneArt, optimized for expression in homo sapiens.| Sequence Annotation | Location | Component / Role(s) |
| N-terminal portion of Cerulean CFP | 1,465 | CDS feature/protein |