His

BBa_K157011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K157011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Kristian M??ller

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:10:54

His affinity tag
Freiburg standard

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K157011_sequence (Version 1)

Description

His-tag; fusion to proteins facilitates detection, purification, immobilization;
NgoMIV / AgeI protein fusion part.

Notes

Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.

Source

Gene synthesis by ATG:biosynthetics, optimized for expression in homo sapiens by iGEM-Team Freiburg 2008

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
His-Tag	1,18	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K157011/1

Member of these Collections

• //proteindomain/affinity
• iGEM Parts Registry