SP-AntiNIP

BBa_K157024 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K157024
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Freiburg 2008
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:10:55

SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_K157024_sequence (Version 1)

Description

A composite part consisting of the signal-peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of split-enzyme beta-Lactamase (intracellular part).

Notes

none

Source

Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002 and BBa_I757011. For further information see the description of the parts mentioned above.

Sequence Annotation Location Component / Role(s)
NgoMI
AgeI
SP-AntiNIP-GGGSlinker-Tm-betaLactamase(Bla1)
SP
Scar (Thr, Gly)
Sc-Fv_anti-NIP
Scar (Thr, Gly)
GGGS-Linker
Scar (Thr, Gly)
Tm
Scar (Thr, Gly)
Bla1
4,9
1480,1485
1,1485
10,81
82,87
88,822
823,828
829,873
874,879
880,948
949,954
955,1479
sequence_feature feature/misc
sequence_feature feature/misc
CDS feature/cds
feature/protein CDS
feature/misc sequence_feature
feature/protein CDS
feature/misc sequence_feature
CDS feature/protein
sequence_feature feature/misc
feature/protein CDS
sequence_feature feature/misc
feature/protein CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K157024/1