SP-AntiNIP

BBa_K157027 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K157027

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM Team Freiburg 2008

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:10:55

SP-AntiNIP-GGGSlinker-Tm-nYFP

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K157027_sequence (Version 1)

Description

Part consists of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP, GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split-fluorophor YFP(intracellular part).

Notes

none

Source

Derived by cloning of the parts BBa_K157001, BBa_K157003, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
NgoMI	4,9	feature/misc, sequence_feature
AgeI	1423,1428	feature/misc, sequence_feature
SP-AntiNIP-GGGSlinker-Tm-nYFP	1,1428	CDS, feature/cds
SP	10,81	CDS, feature/protein
Scar (Thr, Gly)	82,87	feature/misc, sequence_feature
Sc-Fv_anti-NIP	88,822	CDS, feature/protein
Scar (Thr, Gly)	823,828	feature/misc, sequence_feature
GGGS-Linker	829,873	CDS, feature/protein
Tm	880,948	CDS, feature/protein
Scar (Thr, Gly)	874,879	feature/misc, sequence_feature
Scar (Thr, Gly)	949,954	sequence_feature, feature/misc
N-Term_YFP	955,1422	CDS, feature/protein

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K157027/1

Member of these Collections

• //function/reporter/color
• //proteindomain/binding
• //proteindomain/internal
• iGEM Parts Registry