SP-Lipocal

BBa_K157035 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K157035

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: iGEM Team Freiburg 2008

Date created: 2008-10-25 11:00:00

Date modified: 2015-05-08 01:10:55

SP-Lipocalin-GGGSlinker-Tm-nYFP

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K157035_sequence (Version 1)

Description

A composite part consisting of the signal peptide of Erbb1/EGF-receptor, which ensures the transport to the cell membrane scFV-antiNIP, GGGSflexlinker (extracellular parts), the trans-membrane region of the Erbb1/EGF-receptor and the N-terminal part of the split fluorophor YFP(intracellular part).

Notes

none

Source

Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002, BBa_K157008. For further information see the description of the parts mentioned above.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SP-Lipocalin-GGGSlinker-Tm-nYFP	1,1215	CDS, feature/cds
NgoMI	4,9	feature/misc, sequence_feature
SP	10,81	feature/protein, CDS
Scar (Thr, Gly)	82,87	sequence_feature, feature/misc
Lipocalin	88,609	CDS, feature/protein
Scar (Thr, Gly)	610,615	sequence_feature, feature/misc
GGGS-Linker	616,660	CDS, feature/protein
Scar (Thr, Gly)	661,666	feature/misc, sequence_feature
Tm	667,735	feature/protein, CDS
Scar (Thr, Gly)	736,741	feature/misc, sequence_feature
N-Term_YFP	742,1209	feature/protein, CDS
AgeI	1210,1215	feature/misc, sequence_feature
BBa_K157035	1,1218	engineered_region, feature/BioBrick

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K157035/1

Member of these Collections

• //function/reporter/fluorescence
• //proteindomain/internal
• iGEM Parts Registry