SP-Lipocal

BBa_K157039 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K157039
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: iGEM Team Freiburg 2008
Date created: 2008-10-25 11:00:00
Date modified: 2015-05-08 01:10:55

SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2



Types
DnaRegion

Roles
Protein_Domain

polypeptide_domain

Sequences BBa_K157039_sequence (Version 1)

Description

A composite part consisting of the signal peptide of Erbb1/EGF-receptor, scFV-antiNIP, GGGSflexlinker (extracellular part), the trans-membrane region of the Erbb1/EGF-receptor and the C-terminal part of the split fluorophor YFP with a fluolinker joined to its N-Terminus (intracellular part).

Notes

none

Source

Derived by cloning of the parts BBa_K157001, BBa_K157004, BBa_K157013, BBa_K157002 and the second half of split Luciferase For further information see the description of the parts mentioned above.

Sequence Annotation Location Component / Role(s)
NgoMI
AgeI
SP-Lipocalin-GGGSlinker-Tm-Splitluciferase1/2
SP
Scar (Thr, Gly)
Lipocalin
Scar (Thr, Gly)
GGGS-Linker
Scar (Thr, Gly)
Tm
Scar (Thr, Gly)
Splitluciferase1/2
4,9
970,975
1,975
10,81
82,87
88,609
610,615
616,660
661,666
667,735
736,741
742,969
sequence_feature feature/misc
sequence_feature feature/misc
feature/cds CDS
feature/protein CDS
feature/misc sequence_feature
CDS feature/protein
feature/misc sequence_feature
CDS feature/protein
sequence_feature feature/misc
CDS feature/protein
sequence_feature feature/misc
feature/protein CDS
igem#experience
None
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K157039/1