Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-08 04:50:17
Linker in fusion protein
| Types | DnaRegion |
| Roles | restriction_enzyme_assembly_scar Scar |
| Sequences | BBa_K1583015_sequence (Version 1) |
Description
A linker with the protein sequence GSGGSG was placed in between the CsgA and the affinity tag for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials fromengineered curli nanofibres"[1]
1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
Notes
We followed the design of "Programmable biofilm-based materials from engineered curli nanofibres"[1]1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
Source
The DNA was synthesized. The sequence of CsgA originates from the genomic DNA of E. coli K-12 MG1655.(http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper "Programmable biofilm-based materials fromengineered curli nanofibres"[1]
1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.