BBa_K1583053

BBa_K1583053 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1583053
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-03 11:00:00
Date modified: 2015-09-18 07:32:56

Non-coding scar site in between promoter and RBS



Types
DnaRegion

Roles
Scar

restriction_enzyme_assembly_scar

Sequences BBa_K1583053_sequence (Version 1)

Description

CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA.

Notes

We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.

Source

The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655.
The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.

Sequence Annotation Location Component / Role(s)
Scar
1,18
feature/scar
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1583053/1