Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-03 11:00:00
Date modified: 2015-09-18 08:03:55
Non-coding scar site in between RBS and gene
| Types | DnaRegion |
| Roles | Scar restriction_enzyme_assembly_scar |
| Sequences | BBa_K1583054_sequence (Version 1) |
Description
CsgC is positively involved in the extracellular aggregation of CsgA into amyloid nanowires (curli assembly) in E.coli functioning as a chaperone for CsgA.Notes
We inserted non-coding scar sequences after the promoter and RBS to prevent missing initial codons during transcription/translation.Source
The RBS and CsgC were synthesized from the genomic sequence of E.coli K-12 MG1655.The promoter is part of the constitutive promoter family made by John Anderson from iGEM Berkeley 2006.