BBa_K1585116

BBa_K1585116 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1585116

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Tobias Schwanemann

Date created: 2015-08-31 11:00:00

Date modified: 2015-09-01 02:19:47

Anderson Promoter with lacI binding site

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1585116_sequence (Version 1)

Description

Promoters of the Anderson Promoter Collection with lacI binding site derived from R_0010 to make them IPTG iducible.

Notes

We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.

The part is shipped in backbone J61002 because the functionality is directly visible by adding IPTG. RFP (E1010) should get expressed then.

Source

Assambly of J23103 and lacI-binding-site of R_0010

The "lacI binding site" of Part R_0010 differs from lacO from Part BBa_J33207.

Components

Access	Instance	Definition
public	BBa_J23116	BBa_J23116
public	BBa_K1585999	BBa_K1585999

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_J23116	1,35	BBa_J23116
BBa_K1585999	44,78	BBa_K1585999

Other Properties

• igem#experience: Fails
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1585116/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //promoter/anderson
• //regulation/constitutive
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry