Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Osthege
Date created: 2015-08-22 11:00:00
Date modified: 2015-08-23 02:08:19
Glycogen synthase GlgA from E. coli K12
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1585300_sequence (Version 1) |
Description
The glycogen synthase synthesizes alpha-1,4-glucan chains using ADP-glucose.The BioBrick was created from the genomic sequence of glgA in E. coli K12.
Six illegal restriction sites were removed to ensure full BioBrick compability.
Notes
Six illegal restriction sites were removed to ensure full BioBrick compability. The native stop codon was replaced with TAATAA.Source
The BioBrick was created from the genomic sequence of glgA in E. coli K12.| Sequence Annotation | Location | Component / Role(s) |
| glgA Replaced G with C to remove AviII site Replaced G with C to remove AvrII site Replaced C with T to remove EcoRI site Replaced G with A to remove PvuII site Replaced G with A to remove PstI site Replaced T with C to remove AgeI site | 1,1437 162,162 405,405 573,573 969,969 1005,1005 1197,1197 | feature/cds CDS feature/s_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation feature/s_mutation point_mutation point_mutation feature/s_mutation |