BBa_K1585300

BBa_K1585300 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1585300
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Michael Osthege
Date created: 2015-08-22 11:00:00
Date modified: 2015-08-23 02:08:19

Glycogen synthase GlgA from E. coli K12



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1585300_sequence (Version 1)

Description

The glycogen synthase synthesizes alpha-1,4-glucan chains using ADP-glucose.

The BioBrick was created from the genomic sequence of glgA in E. coli K12.

Six illegal restriction sites were removed to ensure full BioBrick compability.

Notes

Six illegal restriction sites were removed to ensure full BioBrick compability. The native stop codon was replaced with TAATAA.

Source

The BioBrick was created from the genomic sequence of glgA in E. coli K12.

Sequence Annotation Location Component / Role(s)
glgA
Replaced G with C to remove AviII site
Replaced G with C to remove AvrII site
Replaced C with T to remove EcoRI site
Replaced G with A to remove PvuII site
Replaced G with A to remove PstI site
Replaced T with C to remove AgeI site
1,1437
162,162
405,405
573,573
969,969
1005,1005
1197,1197
feature/cds CDS
feature/s_mutation point_mutation
feature/s_mutation point_mutation
feature/s_mutation point_mutation
feature/s_mutation point_mutation
feature/s_mutation point_mutation
point_mutation feature/s_mutation
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1585300/1