BBa_K1587006

BBa_K1587006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1587006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Alexandre Le Scornet

Date created: 2015-09-13 11:00:00

Date modified: 2015-09-18 03:05:33

pompC and lacI box regulated cI expression

Details

• Types: DnaRegion
• Roles: engineered_region; Device
• Sequences: BBa_K1587006_sequence (Version 1)

Description

Uses a positively regulated promoter (pompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by cph8 red light receptor (cph1-envZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes.

Notes

No information

Source

aa

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pompC	1,108	promoter, feature/promoter
lacI binding site	109,140	feature/operator, operator
rbs	141,155	feature/rbs, ribosome_entry_site
cI	156,905	CDS, feature/protein

Other Properties

• igem#experience: Issues
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1587006/1

Member of these Collections

• //chassis/prokaryote/ecoli
• iGEM Parts Registry