BD-CRY2

BBa_K1592005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1592005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shuyan Tang

Date created: 2015-09-02 11:00:00

Date modified: 2015-09-18 09:25:52

GalBD-CRY2 Fusion for Yeast-Two-Hybrid

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1592005_sequence (Version 1)

Description

The light-induced transcriptional control is based on a blue light stimulated photoreceptor, cryptochrome 2 (CRY2) and its interacting partner, CIB1. The CRY2/CIB1 interaction is entirely genetically encoded and does not require addition of any exogenous cofactors. The binding naturally reverses within minutes in the dark, allowing rapid shutoff of transcription by placing samples in the dark.
This system is based on yeast two-hybrid interaction, in which a light-mediated protein interaction brings together two halves (a binding domain, GalBD and an activation domain, GAL4AD) of a split transcription factor.

Notes

No

Source

Plasmid from Addgene.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Gal Binding Domain	1,444	CDS, feature/protein
CRY2	457,1956	CDS, feature/protein

Other Properties

• igem#experience: Works
• igem#status: Planning
• synbiohub#ownedBy: user/myers
• synbiohub#ownedBy: user/james
• synbiohub#topLevel: BBa_K1592005/1

Member of these Collections

• iGEM Parts Registry