Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuyan Tang
Date created: 2015-09-03 11:00:00
Date modified: 2015-09-17 07:12:43
GalAD-CIB1 Fusion for Yeast-Two-Hybrid
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1592006_sequence (Version 1) |
Description
CIB1, a basic helix-loop-helix (bHLH) protein, would interact with cryptochrome 2 (CRY2), a blue light stimulated photoreceptor, when exposed to blue light. The CRY2/CIB1 interaction is entirely genetically encoded and does not require addition of any exogenous cofactors. These modules require no exogenous chromophore, are reversible within minutes, trigger protein translocation on a sub-second time scale, and even allow potential use in vivo in whole organisms.This fusion protein is for use in a yeast-two-hybrid system, and a Gal4 DNA activating domain fused to its C terminus.
To regulate DNA transcription by blue light, the system is based on a two-hybrid interaction in which a light-mediated protein interaction brings together two halves (a binding domain and an activation domain) of a split transcription factor. If we remove the stimulation of blue light, dark reversion of CRY2 will dissociate the interaction with CIB1 and halt Gal4-dependent transcription.
Notes
NoSource
Plasmid from Addgene.| Sequence Annotation | Location | Component / Role(s) |
| Gal Activating Domain CIB1 | 1,408 457,1460 | CDS feature/protein CDS feature/protein |