BBa_K1592020

BBa_K1592020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1592020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shuyan Tang

Date created: 2015-09-08 11:00:00

Date modified: 2015-09-16 08:47:12

Ptrp mutant1

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K1592020_sequence (Version 1)

Description

ptrp is a promoter with the trp operator. It will be repressed by trpR (K588000) and LovTAP.
Note that the original trp operator contains two SpeI sites。
We remove the illegal sites without affecting its function by site-directed mutagenesis for meeting the requirements of RFC10 and more convenient to be assembled, and finally constructed three ptrp muant, called ptrp mutant1, ptrp mutant2, and ptrp mutant3.

Notes

none

Source

Cloned from BBa_K588001 and mutated by overlap extension PCR.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
T-G	45,45	feature/mutation, sequence_alteration
A-C	54,54	feature/mutation, sequence_alteration

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1592020/1

Member of these Collections

• iGEM 2018 Distribution Plate 7
• iGEM 2018 Distribution Plate 7 Well 3J
• iGEM 2018 Part Library
• iGEM 2018 Plasmid pSB1C3
• iGEM Parts Registry