BBa_K1595029

BBa_K1595029 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1595029
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jonlin Chen
Date created: 2015-09-12 11:00:00
Date modified: 2015-09-18 01:47:33

EDA and GSG linker



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1595029_sequence (Version 1)

Description

Coding device for KDPG aldolase (EDA), a peptide-stabilizing protein, and a GSG linker sequence.

Notes

Use in conjunction with strain-expressing T7 polymerase.

Added GSG so EDA could easily be linked to it's associated peptide in future use.

Source

Synthesized by DNA 2.0

Sequence Annotation Location Component / Role(s)
Terminator
6xHist tag
NdeI
BamHI cutsite
GSG linker
EDA
RBS
T7
759,801
741,750
735,740
729,734
720,728
81,719
22,80
1,21
feature/misc sequence_feature
tag feature/tag
feature/misc sequence_feature
sequence_feature feature/misc
CDS feature/cds
feature/protein CDS
ribosome_entry_site feature/rbs
feature/promoter promoter
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1595029/1