Submit
Login or Register
View
BBa_K1598004
BBa_K1598004
Version 1
Component
Source:
http://parts.igem.org/Part:BBa_K1598004
Generated By:
https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Napiorkowska
Date created: 2015-08-26 11:00:00
Date modified: 2015-08-27 03:11:46
RBS-GAD-6xHis-Terminator
Download
Download SBOL File
Download COMBINE Archive
Download GenBank
Download GFF3
Download FASTA
Download Image
Search
Find Uses
Find Twins
Send
Send to Benchling
Send to ICE
Back
Details
Types
DnaRegion
Roles
engineered_region
Generator
Sequences
BBa_K1598004_sequence (Version 1)
Description
This part consists of
B0034 RBS
, human glutamate decarboxylase (GAD) gene with C-terminal His tag, and
B0015 terminator
.
Notes
- human CHAT gene (UniProt accession no: Q99259) was codon-optimized for E.coli and provided with C-terminal 6xHis tag
- illegal restriction enzyme sites were removed
- part was obtained through de novo DNA synthesis
Source
DNA synthesis
Sequence Annotations
Sequence Annotation
Location
Component
/ Role(s)
RBS B0034
GAD
Terminator B0015
1,12
19,1440
1447,1575
feature/rbs
ribosome_entry_site
CDS
feature/cds
feature/stop
stop_codon
Other Properties
igem#status
Planning
synbiohub#ownedBy
user/james
synbiohub#ownedBy
user/myers
synbiohub#topLevel
BBa_K1598004/1
Member of these Collections
iGEM Parts Registry
Attachments
Component Sankey
616beaa9-d0e2-419f-b9f6-d4bbfdac536b
iGEM Main
c973bf1b-e0de-4f38-91f7-a8aaca11e3df
iGEM Design
cb76ef85-c614-4571-ad25-4b9a61a3550d
iGEM Experience
7f019bdf-825e-485b-b00e-3ec7a69be099
Bar
3f48e2fa-2a7c-406d-9f23-95efe6eab52d