Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Yash Mishra
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-21 03:58:55
J23101 promoter + RBS + Human FDXR + RBS + Human FDX1 + RBS + Human CYP11A1 + rrnb double terminator
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1598008_sequence (Version 1) |
Description
Also called Cytochrome P450scc, Cholesterol Side Chain Cleavage Enzyme (P450scc) is a mitochondrial enzyme that converts cholesterol into pregnenolone, which is a neurosteroid as well as the precursor to a variety of steroid hormones and neurotransmitters. The gene that encodes it is CYP11A1. Furthermore, the catalysis involves 3 monooxygenase reactions, which requires two electron transport proteins: ferrodoxin (FDX1) and ferredoxin reductase (FDXR). Our biobrick consists of the iGEM prefix followed by the J23101 constitutive promoter, RBS, FDXR gene, RBS, FDX1 GENE, RBS, CYP11A1 gene, rrnb double terminator, and the iGEM suffix. This will be ligated onto a PSB1C3 backbone.Emerging preclinical and clinical evidence suggests that pregnenolone may be a promising novel therapeutic candidate in schizophrenia. It has also been shown to reduce the side effects of benzodiazepines. Furthermore, cholesterol side chain cleavage is the 1st step in the biosynthesis of steroids such as testosterone, progesterone, cortisol, estrogen, etc. Therefore, this biobrick has a plethora of applications, and is in a way several biobricks in 1.
Notes
We had to make sure that that the genes for Cytochrome P450scc and all it's electron transport proteins were included in the biobrick and came after ribosome binding sites. The biobrick was broken into 5 parts for quick synthesize and assembled using Golden Gate assembly. Hence, each part was made compatible for golden gate assembly, and had overhangs for SapI.Source
http://www.ncbi.nlm.nih.gov/nuccore/153218645?report=fastahttp://www.ncbi.nlm.nih.gov/nuccore/16877631?report=fasta
http://www.ncbi.nlm.nih.gov/nuccore/39645178?report=fasta
http://parts.igem.org/cgi/partsdb/puttext.cgi
http://parts.igem.org/cgi/partsdb/puttext.cgi
| Sequence Annotation | Location | Component / Role(s) |
| Human FDX1 Gene BBa_J23101 Human FDXR Gene BBa_J34801 BBa_J34801 BBa_J34801 Human CYP11A1 Gene rrnb Double Terminator preceded by some random bases | 1562,2117 1,33 58,1534 39,51 1543,1555 2126,2138 2151,3716 3717,3910 | CDS feature/cds promoter feature/promoter feature/cds CDS ribosome_entry_site feature/rbs feature/rbs ribosome_entry_site feature/rbs ribosome_entry_site feature/cds CDS feature/stop stop_codon |