| Types | DnaRegion
|
| Roles | CDS
Coding
|
| Sequences | BBa_K1600000_sequence (Version 1)
|
Description
This part serves to increase the uptake of glucose by the cell. It is designed to work in conjunction with EIIA (BBa_K1508003) or the composite EIIA device (BBa_K1600001), another part of glucose uptake in E. coli. EIIBC, also known as ptsG, is the active channel for glucose uptake in E. coli as a part of the Phosphotransferase System (PTS) so this BioBrick would upregulate the uptake of glucose. While EIIA is not a transmembrane transferase protein, it plays a prominent second step in the PTS pathway (http://spin.niddk.nih.gov/clore/Structures/HPr-IIAMtl/pict.html), the first step being glucose uptake by the transmembrane protein complex EIIBC. We hypothesize that the use of EIIBC with EIIA will increase glucose upake in E. coli.
Notes
None.
Source
PCR-amplified from the XLIBlue E.coli K-12 substrain.
