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BBa_K1603003

BBa_K1603003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1603003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Hellgren

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 06:52:02

RecA - optimized for yeast

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Details

Types	DnaRegion
Roles	Coding CDS
Sequences	BBa_K1603003_sequence (Version 1)

Description

Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms.
Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair

Notes

Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair

Source

Sequence adapted from Deinococcus Radiodurans. Ordered as synthetic gene.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ATG His-Tag SV40 NLS RecA TAA	1,3 13,30 34,54 64,1149 1150,1152	start_codon feature/start feature/misc sequence_feature feature/misc sequence_feature feature/protein CDS stop_codon feature/stop