BBa_K1603003

BBa_K1603003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1603003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Hellgren

Date created: 2015-09-17 11:00:00

Date modified: 2015-09-18 06:52:02

RecA - optimized for yeast

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1603003_sequence (Version 1)

Description

Recombinase A from Deinococcus Radiodurans. Used in DNA-repair mechanisms.
Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair

Notes

Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair

Source

Sequence adapted from Deinococcus Radiodurans. Ordered as synthetic gene.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
ATG	1,3	start_codon, feature/start
His-Tag	13,30	feature/misc, sequence_feature
SV40 NLS	34,54	feature/misc, sequence_feature
RecA	64,1149	feature/protein, CDS
TAA	1150,1152	stop_codon, feature/stop

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1603003/1

Member of these Collections

• iGEM Parts Registry