BBa_K1603004

BBa_K1603004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1603004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: John Hellgren
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-18 06:58:39

SSB - optimized for yeast



Types
DnaRegion

Roles
CDS

Coding

Sequences BBa_K1603004_sequence (Version 1)

Description

Single strand binding protein from Deinococcus Radiodurans. Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair

Notes

Codon optimized for saccharomyces cerevisiae and fused with a nuclear localization signal (NLS) for in vivo DNA repair. Also fused with a His-tag, allowing nickel based purification prior in vitro DNA repair.

Source

Sequence adapted from Deinococcus Radiodurans. Ordered as synthetic gene.

Sequence Annotation Location Component / Role(s)
ATG
His-Tag
SV40 NLS
SSB
TAA
1,3
13,30
34,54
64,966
967,969
start_codon feature/start
feature/misc sequence_feature
feature/misc sequence_feature
feature/protein CDS
feature/stop stop_codon
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1603004/1