BBa_K1607001

BBa_K1607001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1607001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiazheng Xing
Date created: 2015-08-04 11:00:00
Date modified: 2015-08-05 10:23:03

HER2 extracellular domain with mcherry



    • Details

    Types
    DnaRegion

    Roles
    Coding

    CDS

    Sequences BBa_K1607001_sequence (Version 1)

    Description

    HER2 extracellular domain linked with mcherry by a (Gly4Ser)3 linker. It can be used in FRET analysis.

    Notes

    A flexible linker is added between the HER2 extracellular domain and the mcherry by SOE PCR.

    Source

    HER2 extracellular domain from tumour cell line SKBR-3 CDNA and mcherry from Biobrick.

    Sequence Annotation Location Component / Role(s)
    HER2-ECD
    Linker
    mcherry
    		1,1935
    1936,1980
    1981,2688
    		feature/cds CDS
    feature/cds CDS
    CDS feature/cds

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1607001/1
     

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