BBa_K1607001

BBa_K1607001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1607001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jiazheng Xing
Date created: 2015-08-04 11:00:00
Date modified: 2015-08-05 10:23:03

HER2 extracellular domain with mcherry



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K1607001_sequence (Version 1)

Description

HER2 extracellular domain linked with mcherry by a (Gly4Ser)3 linker. It can be used in FRET analysis.

Notes

A flexible linker is added between the HER2 extracellular domain and the mcherry by SOE PCR.

Source

HER2 extracellular domain from tumour cell line SKBR-3 CDNA and mcherry from Biobrick.

Sequence Annotation Location Component / Role(s)
HER2-ECD
Linker
mcherry
1,1935
1936,1980
1981,2688
feature/cds CDS
feature/cds CDS
CDS feature/cds
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1607001/1