Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Frieda Anna Sorgenfrei
Date created: 2015-09-15 11:00:00
Date modified: 2015-09-16 04:49:44
Spinach in BBF RFC 110 transcription cassette
| Types | DnaRegion |
| Roles | mature_transcript_region RNA |
| Sequences | BBa_K1614011_sequence (Version 1) |
Description
The Spinach II aptamer in RFC 110 cloning standard for in vitro transcription and in vivo expression.Notes
Cloned without restriction enzymes to avoid issues occurring from conserved cut sites. For more details see RFC 110.Source
Synthetic (Babendure, J.R., Adams, S.R., and Tsien, R.Y. (2003). Aptamers switch on fluorescence of triphenylmethane dyes. Journal of the American Chemical Society 125, 14716-14717.)| Sequence Annotation | Location | Component / Role(s) |
| T7 Promotor Spinach II HDV G required for transcription | 13,29 31,124 125,208 30,30 | feature/promoter promoter feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc |