BBa_K1615020

BBa_K1615020 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1615020

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brooke Rothschild-Mancinelli

Date created: 2015-08-26 11:00:00

Date modified: 2015-08-27 05:19:10

Linker-CBDcipA-linker fused to MorA driven by LacI promoter

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1615020_sequence (Version 1)

Description

Morphine-6-dehydrogenase fused C terminally to CBDcipA with N and C terminal linkers driven by the IPTG inducible PLlac 0-1 promoter.

Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.

Source

Morphine-6-dehydrogenase from Pseudomonas putida. CBDcipA from Clostridium thermocellum. Lac expression cassette from iGEM10_Washington.

Components

Access	Instance	Definition
public	BBa_K314103	BBa_K314103
public	BBa_K1319106	Scar25 ATG
public	BBa_K1615010	BBa_K1615010

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K314103	1,1638	BBa_K314103
BBa_K1319106	1639,1653	Scar25 ATG
BBa_K1615010	1654,3252	BBa_K1615010

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1615020/1

Member of these Collections

• iGEM Parts Registry