BBa_K1615104

BBa_K1615104 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1615104

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brooke Rothschild-Mancinelli

Date created: 2015-08-30 11:00:00

Date modified: 2015-08-31 05:03:32

mRFP fused to Linker+dCBD driven by LacI promoter

Details

• Types: DnaRegion
• Roles: Composite; engineered_region
• Sequences: BBa_K1615104_sequence (Version 1)

Description

mRFP fused N terminally to dCBD + N terminal linker driven by the IPTG inducible PLlac 0-1 promoter

Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.

Source

mRFP highly engineered variant originally from Discosoma striata. dCBD from Trichoderma reesei. Lac expression cassette from iGEM10_Washington.

Components

Access	Instance	Definition
public	BBa_K314103	BBa_K314103
public	BBa_K1319106	Scar25 ATG
public	BBa_K1615094	BBa_K1615094

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K314103	1,1638	BBa_K314103
BBa_K1319106	1639,1653	Scar25 ATG
BBa_K1615094	1654,2700	BBa_K1615094

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1615104/1

Member of these Collections

• iGEM Parts Registry