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BBa_K1615108

BBa_K1615108 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1615108

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brooke Rothschild-Mancinelli

Date created: 2015-08-30 11:00:00

Date modified: 2015-08-31 05:14:41

mRFP fused to CBDclos driven by LacI promoter

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Details

Types	DnaRegion
Roles	engineered_region Composite
Sequences	BBa_K1615108_sequence (Version 1)

Description

mRFP fused N terminally to CBDclos driven by the IPTG inducible PLlac 0-1 promoter.

Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.

Source

mRFP highly engineered variant originally from Discosoma striata. CBDclos from Clostridium cellulovorans. Lac expression cassette from iGEM10_Washington.

Components

Access	Instance	Definition
public public public public public public	BBa_K314103 BBa_K1319106 BBa_K1615089 BBa_B0105 BBa_K1321002 BBa_K1615098	BBa_K314103 Scar25 ATG mRFP Scar 25 BBa_K1321002 BBa_K1615098

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K314103 BBa_K1319106 BBa_K1615089 BBa_B0105 BBa_K1321002 BBa_K1615098	1,1638 1639,1653 1654,2328 2329,2334 2335,2634 1654,2634	BBa_K314103 Scar25 ATG mRFP Scar 25 BBa_K1321002 BBa_K1615098