BBa_K1615109

BBa_K1615109 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1615109

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Brooke Rothschild-Mancinelli

Date created: 2015-08-30 11:00:00

Date modified: 2015-08-31 05:17:50

Linker-CBDcipA-linker fused to mRFP driven by LacI promoter

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K1615109_sequence (Version 1)

Description

mRFP fused C terminally to CBDcipA with N and C terminal linkers driven by the IPTG inducible PLlac 0-1 promoter.

Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.

Source

mRFP highly engineered variant originally from Discosoma striata. CBDcipA from Clostridium thermocellum. Lac expression cassette from iGEM10_Washington.

Components

Access	Instance	Definition
public	BBa_K314103	BBa_K314103
public	BBa_K1319106	Scar25 ATG
public	BBa_K1615099	BBa_K1615099

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K314103	1,1638	BBa_K314103
BBa_K1319106	1639,1653	Scar25 ATG
BBa_K1615099	1654,3045	BBa_K1615099

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1615109/1

Member of these Collections

• iGEM Parts Registry