Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Johanna Chesnel
Date created: 2015-09-16 11:00:00
Date modified: 2015-09-22 06:42:02
VVD linked to YC155 (YFP Cter split) with promoter T7
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1616001_sequence (Version 1) |
Description
Vivid (VVD) is the smallest known Light???oxygen???voltage (LOV) domain protein and photo-inducible dimer. Isolated from Neurospora crassa, VVD forms a homo-dimer in response to a blue-light stimulus.Then, a split protein is a protein whose sequence has been divided into two (or more) different parts. The yellow-fluorescent (YFP) protein will only express fluorescence when its two parts will be reunited.
The Part is coding for the homodimer VVD link by an integration of specific sequence to the C terminal of the YFP split.
So, this part works with BBa_K1616002. In absence of blue-light, the conformation of the VVD photoreceptor will prevent the formation of the complete fluorescent protein while in presence of the light signal the YFP protein will be reconstituted leading to the fast expression of a yellow fluorescence in our bacteria.
Notes
The sequence of VVD had 2 illegal sites PstI; that have been removed.Source
This part have been created thank to gblock, our team have assembled the sequence of photoreceptor VVD (without illegal site), a linker(1) and then the C terminal of YFP split(1).(1) Tom Kerppola, Ph. D, investigator at the Howard Hughes Medical Institute as well as Professor in the University of Michigan
| Access | Instance | Definition |
| public public public | BBa_I712074 BBa_J61100 BBa_K1616021 | BBa_I712074 BBa_J61100 VVD-YC |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_I712074 BBa_J61100 BBa_K1616021 | 1,46 55,66 75,830 | BBa_I712074 BBa_J61100 VVD-YC |