Types | DnaRegion
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Roles | CDS
Coding
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Sequences | BBa_K1616023_sequence (Version 1)
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Description
NanoLuc (Nluc) is a novel engineered luciferase coupled with a novel coelenterazine analogue: Furimazine. When both are coupled, we obtain a much brighter luminescence than Fluc or Rluc and it is known to work exceptionally well as a reporter.
NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.
Thermal stable enzyme
NanoLuc retains activity following 30 min incubation at 55 ??C.
Active over broad pH range
NanoLuc is fully active between pH 7-9 and retains significant activity at pH 5-7.
Monomeric enzyme
NanoLuc facilitates use as transcriptional reporter or fusion partner.
Notes
Presence of XbaI site.
Source
NanoLuc is a 19.1 kDa enzyme which is an ATP independent luciferase providing a glow-type luminescence.