Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Ramona Emig, Rabea Jesser, Julika Neumann, Lara Stuehn
Date created: 2015-08-27 11:00:00
Date modified: 2015-08-30 09:01:17
Glycoprotein E1 epitopes derived from Rubella Virus
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1621000_sequence (Version 1) |
Description
The Rubella Virus is the only member of the genus of Rubivirus. It contains single-stranded positive-sense RNA that encodes three structural and two non-structural proteins. Two of the structural proteins are the envelope proteins E1 and E2 which form heterodimers that are arranged in groups of three all over the viruses surface. In our project we express the protein E1 that was shown to provoke an immunogenic response and to have ???hemagglutinating and virus-neutralizing activities??? (e.g. Gillam et al.: Cellular and Humoral Immune Responses to Rubella Virus Structural Proteins E1, E2 and C).The virus is transmitted via airborne infection with humans as the only known hosts. It causes the rubella disease, also known as German measles or Rubeola. After infection the virus persists in the body for the whole life providing lifelong immunity and can therefore be referred to as a typical children???s disease. The incubation time amounts to 14 to 21 days and goes along with exanthemas, fever, headache, rheumatic pains and swollen lymph nodes. The patient is contagious from one week before the first exanthema until one week after the last one. The infection can also be asymptomatic for some patients as opposed to possible complications in rare incidents. Arthritis, bronchitis, encephalitis and inclusion of heart damages are conceivable in such cases. As the virus can also cross the placenta, pregnant women getting infected in the beginning of their pregnancy might lose their child due to a spontaneous abort. The children can also still be born but might then suffer from deformities of the heart, cataracts or labyrinthine deafness.
Therapies are only symptomatic but there is a vaccination available that could prevent the disease.
Notes
The sequence was codon optimized for expression in E. coli with the IDT codon optimization tool.Source
The sequence was obtained from Best et al. (1995) and synthesized by Integrated DNA Technologies.| Sequence Annotation | Location | Component / Role(s) |
| Glycoprotein E | 4,228 | feature/cds CDS |