Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lucas Krauss
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-20 08:41:50
Glycoaldehyde reductase and dehydrogenase
| Types | DnaRegion |
| Roles | CDS Coding |
| Sequences | BBa_K1622000_sequence (Version 1) |
Description
Our part is the combination of two genes coding for enzymes that belong to the degradation pathway of Ethylene Glycol:- the Glycoaldehyde Reductase (BBa_K936023): this enzyme catalyzes the transformation of Ethylene Glycol into Glycoaldehyde.
- the Glycoaldehyde Dehydrogenase (BBa_K936011): this enzyme catalyzes the reaction from Glycoaldehyde to Glycolate.
We used it as one of our clusters in the PET degradation operon.
Notes
We designed our BioBrick using the sequences provided by the Registry of Standard Biological Parts and optimized these sequences for E.coli.Source
This part is the result of the Gibson Assembly of BBa_K936011 and BBa_K936023. We used the sequence provided in the iGEM registry of standard parts, optimized them for E.coli. We ordered the parts to be synthesized.| Sequence Annotation | Location | Component / Role(s) |
| RBS RBS Glycoaldehyde dehydrogenase Glycoaldehyde reductase | 1469,1482 3,16 24,1466 1490,2641 | ribosome_entry_site feature/rbs feature/rbs ribosome_entry_site engineered_region feature/BioBrick engineered_region feature/BioBrick |