BBa_K1627000

BBa_K1627000 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1627000
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandro Gutierrez
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-11 02:15:10

Device to demethylate 1-methylxanthine



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K1627000_sequence (Version 1)

Description

This part contains genes that should allow for the demethylation of 1-methylxanthine. The part contains a synthetic operon consisting of ndmA, ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.

While this device should be able to demethylate the 1-methyl group on methylxanthines, the ndmA gene lacks specificity to 1-methylxanthine. Thus, this part is not actually able to be used as designed. However, this part was important for the construction of parts BBa_K1627003, BBa_K1627004, and BBa_K1627006, which are able to demethylate the 1-methylgroup from theophylline, paraxanthine, and caffeine, respectively.

Notes

These genes were amplified from the pDCAF3 plasmid (BBa_K734000). A PstI restriction site was removed from the ndmA gene during the cloning process. The genes were put together into the pSB1C3 backbone using BioBrick Standard Assembly.

Source

The ndmA and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille.

Sequence Annotation Location Component / Role(s)
J23110
K515105
ndmA
B0032
ndmD
B0034
gst9
1,35
44,68
69,1124
1133,1145
1152,2918
2963,2974
2981,3655
feature/promoter promoter
ribosome_entry_site feature/rbs
feature/cds CDS
ribosome_entry_site feature/rbs
CDS feature/cds
feature/rbs ribosome_entry_site
CDS feature/cds
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1627000/1