BBa_K1627003

BBa_K1627003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1627003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandro Gutierrez
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-10 04:23:12

Device to demethylate theophylline



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K1627003_sequence (Version 1)

Description

This part contains genes that allow for the demethylation of theophylline. The part contains a synthetic operon consisting of ndmA, ndmB, and ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.

This part can be used in a ∆guaB strain of E. coli to measure the amount of theophylline in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of theophylline, a growth curve can be made that correlates culture growth with concentration of theophylline. Then if the strain is grown in a minimal media with an unknown concentration of theophylline, the growth can be compared to the growth curve to calculate the initial concentration of theophylline in the media.

Notes

These genes were amplified from the pDCAF3 plasmid (BBa_K734000). A PstI restriction site was removed from the ndmA gene and an EcoRI restriction site was removed from the ndmB gene during the cloning process. The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly.

Source

The ndmA, ndmB, and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille.

Sequence Annotation Location Component / Role(s)
J23110
K515105
ndmA
K082001
ndmB
B0032
ndmD
B0034
gst9
1,35
44,68
69,1124
1133,1147
1154,2221
2230,2242
2249,4015
4060,4071
4078,4752
feature/promoter promoter
feature/rbs ribosome_entry_site
feature/cds CDS
feature/rbs ribosome_entry_site
CDS feature/cds
ribosome_entry_site feature/rbs
CDS feature/cds
ribosome_entry_site feature/rbs
CDS feature/cds
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1627003/1