BBa_K1627004

BBa_K1627004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1627004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alejandro Gutierrez
Date created: 2015-09-09 11:00:00
Date modified: 2015-09-17 07:30:00

Device to demethylate paraxanthine



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K1627004_sequence (Version 1)

Description

This part contains genes that allow for the demethylation of paraxanthine. The part contains a synthetic operon consisting of ndmA, ndmC, and ndmD from Pseudomonas putida CBB5 and gst9 from Janthinobacterium sp. Marseille under the control of a constitutive promoter, J23110.

This part can be used in a ∆guaB strain of E. coli to measure the amount of paraxanthine in a solution. By growing the ∆guaB strain with this device in a minimal media with different known concentrations of paraxanthine, a growth curve can be made that correlates culture growth with concentration of paraxanthine. Then if the strain is grown in a minimal media with an unknown concentration of paraxanthine, the growth can be compared to the growth curve to calculate the initial concentration of paraxanthine in the media.

Notes

These genes were amplified from the pDCAF3 plasmid (BBa_K734000). A PstI restriction site was removed from the ndmA gene during the cloning process. The genes were cloned into the pSB1C3 backbone using BioBrick Standard Assembly.

Source

The ndmA, ndmC, and ndmD genes were isolated from Pseudomonas putida CBB5. gst9 was isolated from Janthinobacterium sp. Marseille.

Sequence Annotation Location Component / Role(s)
J23110
K515105
ndmA
B0030
ndmC
B0032
ndmD
B0034
gst9
1,35
44,68
69,1124
1133,1147
1154,2008
2017,2029
2036,3802
3847,3858
3865,4539
feature/promoter promoter
feature/rbs ribosome_entry_site
feature/cds CDS
ribosome_entry_site feature/rbs
CDS feature/cds
feature/rbs ribosome_entry_site
feature/cds CDS
feature/rbs ribosome_entry_site
feature/cds CDS
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1627004/1