Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Devin Wehle
Date created: 2015-09-11 11:00:00
Date modified: 2015-09-17 12:28:57
Yellow Fluorescent Protein, with Medium Promoter and RBS
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1627008_sequence (Version 1) |
Description
This is a plasmid using the pSB1C3 backbone, which is composed of the CamR resistance gene and the pMB1 origin of replication. A vector was inserted into the plasmid backbone,BBa_K608006 (J23110 + B0032), which is composed of the Bio-Brick promoter BBa_J23110 and ribosome binding site BBa_B0032. A fluorescent protein gene was then inserted into the resulting plasmid. BBa_K592101, the Super Yellow Fluorescent Protein gene was the fluorescent gene inserted in the plasmid.This plasmid allows the creation of the yellow fluorescent protein inside bacteria.
This plasmid (BBa_K1627008) was used in the study of stability of genetic devices across generations by the 2015 University of Texas at Austin iGEM team. The plasmid has been observed be remain genetically stable across one-hundred generations. Point mutation in the promoter and IS element inserts were prominent mutations that destabilized the plasmid. Specifically, IS10R and IS10L was a common IS element inserted into the plasmid.
Notes
The plasmid was constructed and cloned from parts from the Biobrick registry.Source
This plasmid was constructed from previously constructed Biobrick parts. The pSB1C3 backbone, BBa_K608006 vector-composed of BBa_J23110 and BBa_ B0032- and the BBa_K592101 Yellow Fluorescent Protein.| Access | Instance | Definition |
| public public public | BBa_J23110 BBa_B0032 BBa_K592101 | BBa_J23110 BBa_B0032 YFP |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_J23110 BBa_B0032 BBa_K592101 | 1,35 44,56 63,782 | BBa_J23110 BBa_B0032 YFP |