Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Parijata Mackey
Date created: 2008-10-10 11:00:00
Date modified: 2015-05-08 01:10:55
Mussel Adhesive Protein Mefp-151
| Types | DnaRegion |
| Roles | CDS Coding |
Description
Protein coding region: Hybrid of the mussel adhesive protein (MAP) fp-151, which is a fusion protein with six type 1 (fp-1) decapeptide repeats at each type 5 (fp-5) terminus.Fp-151-RGD is a fusion with the GRGDSP residues, a RGD peptide sequence that has previously been identified at the cell-attachment site of fibronectin, at the C-terminus of fp-151. Although recombinant fp-151-RGD maintained the advantages associated with fp-151, such as a high production yield in Escherichia coli and simple purification, it showed superior spreading ability, which is important for cell proliferation under serum-free conditions, as well as better cell-adhesion ability compared with other commercially produced cell-adhesion materials such as poly-l-lysine (PLL) and the naturally extracted MAP mixture Cell-Tak. The excellent adhesion and spreading abilities of fp-151-RGD might be due to the fact that it utilizes three types of cell-binding mechanisms: DOPA adhesion of Cell-Tak, cationic binding force of PLL, and RGD sequence-mediated adhesion of fibronectin. Therefore, the new recombinant fp-151-RGD is suitable for use as a cell-adhesion material in cell culture or tissue engineering, and in any other area where efficient cell adhesion is required.
Notes
652bp - a fusion of fp151 and RGD peptide (GRGDSP)(6[{fp-1 decapeptide}])[{Mgfp-5}](6[{fp-1 decapeptides}])[{GRGDSP}]([Stop])