Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Xinhong Chen
Date created: 2015-09-06 11:00:00
Date modified: 2015-09-07 02:24:10
Pmyo2-ChR2-YFP (C.elegans)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K1634000_sequence (Version 1) |
Description
Pmyo2 is a promoter which can drive the expression in the muscle of C.elegans especially the muscle in the head and pharynx. ChR2 is a kind of channelrhodopsin. Channelrhodopsins are a subfamily of retinylidene proteins (rhodopsins) that function as light-gated ion channels.[1] They serve as sensory photoreceptors in unicellular green algae, controlling phototaxis: movement in response to light.[2] When we express the channelrhodopsins in some specific cells in organisms and shed specific light on them, we can activate or supress the specific ion channel to change the activity of the cell. According to this thought in optogenetic, we linked the Pmyo2，specific promoter in the muscle of head, with the ChR2, then we use the light source assembled by ourselves to shed light of specific wave on the C.elegans,changging the condition of the muscle. Since we have learned that the chosing of direction in C.elegans depends on the muscle in the head, we can observe the obvious change in moving pattern of the C.elegans after we shed the light. In other words,we try to construct a light-sensed locomotion controlling system in C.elegans. And we achieve that goal by using this part to control the movement of the head, the movement of the whole C.elegans.References:
1. Nagel G, Ollig D, Fuhrmann M, Kateriya S, Musti AM, Bamberg E, Hegemann P (June 2002). "Channelrhodopsin-1: a light-gated proton channel in green algae". Science 296 (5577): 2395???8. doi:10.1126/science.1072068
2. Sineshchekov OA, Jung KH, Spudich JL (June 2002). "Two rhodopsins mediate phototaxis to low- and high-intensity light in Chlamydomonas reinhardtii". Proc. Natl. Acad. Sci. U.S.A. 99 (13): 8689???94. doi:10.1073/pnas.122243399.