Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Rebecca Buchanan
Date created: 2015-09-17 11:00:00
Date modified: 2015-09-19 08:47:38
BBa_K1635000 (Archaeal translational efficiency part)
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K1635000_sequence (Version 1) |
Description
The ribosome binding sites (RBS) of archaea are not well characterized. By creating and characterizing a library of RBS sequences, researchers will be able to express proteins of interest at variable levels of expression in Methanococcus maripaludis. Ribosome binding sites are typically 6-7 base pair sequences on a transcript that is complementary to the 3??? end of the 16S rRNA. After binding of the RBS to the ribosome, translation will be initiated. An RBS with higher affinity for the ribosome will result in higher rate of translation, and inversely, an RBS with lower affinity will result in lower rate of translation. In addition to altering the RBS sequence, the immediately downstream bases (spacer region) could affect affinity of the ribosome as well. This part has a point mutation in the fourth base of the spacer region.
Notes
We have ensured Bio Brick Compatibility for all our parts.
Source
The PhmvA promoter is a known archaeal promoter, the native RBS sequence was obtained from Methanococcus voltae. The mCherry gene was obtained from Clonentech (Discosoma sp.)and was then codon optimized for expression in Methanococcus.